Rapid and efficient preparation of DNA, proteins, and chromatin is vital in performing downstream analyses. Our suite of sample preparation kits are suitable for nearly any workflow, whether it be isolating chromatin for ChIP-Seq or ChIP-PCR applications or preparing DNA from a variety of starting materials for Bisulfite-Seq or MS-PCR.
Our sample preparation kits enable you to go directly from extraction to reaction, offering countless possibilities and flexibility in your experimental workflow. Our DNA, protein, and chromatin preparation kits are the starting point for endless research opportunities and downstream applications.
No matter what assay you may be carrying out, proper sample preparation is the initial step that can dictate the success of an entire experiment. EpiGentek's sample preparation products place particular emphasis on the following features: (a) rapid isolation of high quality, ready-to-use DNA, protein, or chromatin samples; (b) efficient yield from trace samples; (c) high flexibility in starting materials; (d) compatibility with various downstream assays or analysis techniques; and (e) specific optimization for use with EpiGentek's assay products.
DNA samples can be prepared from cells, tissues, and body fluids, and are the most commonly used starting material for the majority of DNA methylation applications and assays. Efficient, precise, and high quality isolation of DNA is a critical precursor to having a successful DNA-based experiment.
Excellent protein preparation and purification protocols are vital in preparing high quality starting materials for the characterization of the function, structure, and interactions of proteins of interest. Both nuclear and histone proteins can be used for a variety of downstream studies, including enzymatic analysis and histone analysis.
Chromatin is a combination of DNA and proteins that packages DNA into a smaller volume to fit in the cell, helps to prevent DNA damage, and controls gene expression and DNA replication. Proper isolation of chromatin samples is an important factor in assays for studying protein-DNA interactions.
Our RNA preparation kits are suitable for the quick and efficient isolation of total RNA directly from samples such as tissue, cultured cells, and cell-free liquid specimens (specifically saliva and nasopharyngeal swabs). The purified RNA can be used for various routine applications, such as RT-PCR, cDNA synthesis, Northern blotting, differential display, primer extension, and mRNA selection.