秒速飞艇网上投注 RNA methylation is a reversible post-translational modification to RNA that epigenetically impacts numerous biological processes. It occurs in different RNAs including tRNA, rRNA, mRNA, tmRNA, snRNA, snoRNA, miRNA, and viral RNA. Different catalytic strategies are employed for RNA methylation by a variety of RNA-methyltransferases. N6-methyladenosine (m6A) is the most common and abundant methylation modification in RNA molecules present in eukaryotes and accounts for more than 80% of all RNA methylation. It may be referred to as the “fifth RNA base” and has broad roles in regulating embryonic development and cell fates. In particular, this RNA modification is thought to be important for mRNA regulation due to enrichment of m6A at 3’UTRs. It has also been linked to cancers including leukemia, breast cancer, and prostate cancer. N6-methyladenosine (m6A) 5-methylcytosine (5-mC) is an epigenetic mark that also commonly occurs in various RNA molecules. Researchers are able to bisulfite convert RNA, perform RT-PCR amplification, cloning, and sequencing to gain information about RNA cytosine methylation states. By treating RNA with bisulfite, cytosine residues are deaminated to uracil while leaving 5-methylcytosine intact. Recent data strongly suggest that m6A and 5-mC RNA methylation affects the regulation of various biological processes such as RNA stability and mRNA translation, and that abnormal RNA methylation contributes to etiology of human diseases. Furthermore, loss of 5-mC in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Researchers continue to investigate m6A and 5-mC in RNA to uncover the mysteries of RNA methylation.   m6A RNA Methylation Quantification KitCat #P-9005 5-mC RNA Methylation ELISA Easy Kit Cat #P-9009 RNA Bisulfite Conversion KitCat #P-9003   Citations Citations Citations Purpose An ELISA-like format for absorbance-based quantitation of N6-methyladenosine in RNA An ELISA-like format for fluorescence-based quantitation of 5-mC RNA methylation For robust, chemical-based bisulfite treatment of RNA for analysis of methylated cytosines Key Points Detect m6A RNA methylation in any species Colorimetric ELISA-like assay using an m6A antibody Detection limit as low as 10 pg of m6A Large signal window, low background noise Detect 5-mC RNA methylation in any species High specificity to 5-mC, with no cross-reactivity Input as low as 5 ng per reaction >90% of RNA loss prevented Completely converts unmethylated RNA cytosine into uracil  Starting Material Total RNA Total RNA Total RNA Lowest Input Amount 50 pg 50-300 ng 200-500 ng Total Protocol Time < 4 hours < 3 hours 3 hours Reactions 48,96 48,96 50 Vessel Format 96-Well Plate 96-Well Plate Spin Column

RNA Methylation Activity/Inhibition