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Methylated DNA immunoprecipitation (meDIP) is a large scale antibody-based technique that is used to enrich and capture methylated DNA fragments for use in gene-specific DNA methylation studies on a genome wide scale. Following meDIP approaches, DNA methylation can be analyzed using a variety of downstream applications including meDIP-PCR, meDIP-ChIP, and meDIP-sequencing.

	Methylamp Methylated DNA Capture (MeDIP) KitCat #P-1015	EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) KitCat #P-1038	EpiQuik MeDIP Ultra KitCat #P-1052	EpiQuik Methylated DNA Immunoprecipitation KitCat #P-2019	EpiQuik Tissue Methylated DNA Immunoprecipitation KitCat #P-2020
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Purpose	Enrich and capture methylated DNA fragments	Enrich and capture hydroxymethylated DNA fragments	Enrich and capture methylated DNA fragments from low starting input	Enrich and capture methylated DNA fragments directly from cell lysates	Enrich and capture methylated DNA fragments directly from tissues
Key Points	Ideal for PCR and microarray Illumina workflow compatible	Compatible with downstream workflows including hMeDIP-PCR and hMeDIP-chip	Ideal for MeDIP-Seq Illumina workflow compatible Most sensitive and specific	Eliminates DNA isolation step by immunoprecipitating meDNA fragments directly from cells	Eliminates DNA isolation step by immunoprecipitating meDNA fragments directly from tissues
Starting Material	DNA	DNA	DNA	Cells	Tissues
Lowest Input Amount	0.2-1 µg	0.1 µg	50 ng DNA (10,000 cells)	100,000 cells	10 mg
Total Protocol Time	< 3 hours	< 3 hours	< 3 hours	< 4 hours	< 3.5 hours
Enrichment Efficiency	> 98%	> 99%	> 99%	> 95%	> 95%
Conversion Efficiency	99.9%	99.9%	99.9%	99.9%	99.9%
Highly Specific Antibody Included
Columns for DNA Purification Included
Vessel Format	96-Well Plate	96-Well Plate	96-Well Plate	96-Well Plate	96-Well Plate