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Chromatin immunoprecipitation (ChIP) is an antibody-based method used for determining the location of DNA binding sites on the genome for a particular protein of interest. This technique is a convenient means for studying protein-DNA interactions that occur inside the nucleus of cells and for understanding cellular processes. Downstream applications of ChIP include ChIP-sequencing, ChIP-PCR, and ChIP-on-chip (microarrays).

	ChromaFlash High Sensitivity ChIP Kit Cat #P-2027	EpiQuik ChIP Kit Cat #P-2002	EpiQuik Tissue ChIP Kit Cat #P-2003	EpiQuik Plant ChIP Kit Cat #P-2014	ChromaFlash One-Step Magnetic ChIP Kit Cat #P-2026
Purpose	Selective immunoprecipitation and enrichment of chromatin fractions from low amount of cells or tissue	Selective immunoprecipitation and enrichment of chromatin fractions from cells	Selective immunoprecipitation and enrichment of chromatin fractions from tissue	Selective immunoprecipitation and enrichment of chromatin fractions from plants	Immunoprecipitate and shear isolated chromatin using magnetic beads in one step
Key Points	Recommended for next-gen sequencing applications	Robust protocol designed specifically for cell input	Robust protocol designed specifically for tissue input	Robust protocol designed specifically for plant tissue input	Magnetic beads-based ChIP allows for automation
Starting Material	Cells or Tissue	Cells	Tissue	Plant Tissue (flowers, leaves, young seedlings)	Isolated Chromatin
Input Range	2,000 to 1,000,000 cells or 0.5 to 50 mg tissue per reaction	500,000 to 2,000,000 cells per reaction	10 to 30 mg tissue per reaction	20 to 50 mg tissue per reaction	0.1 to 15 µg chromatin per reaction
Total Protocol Time	5 hours	5 hours	5 hours	6 hours	4 hours
Relative-Fold Enrichment vs IgG	500+	100 to 200	100 to 200	100 to 200	100 to 200
DNA Purification Columns Included					Not needed
Negative Control Included
Control Primers Included				Not needed
Vessel Format	Strip-Well Microplate	Strip-Well Microplate	Strip-Well Microplate	Strip-Well Microplate	Strip-Well Microplate
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